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human recombinant il 1α  (R&D Systems)


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    R&D Systems human recombinant il 1α
    Human Recombinant Il 1α, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+il+1a/Recombinant+Human+IFN-alpha-1a+Protein%2C+CF/us12582628-101-3-8
    Average 94 stars, based on 28 article reviews
    human recombinant il 1α - by Bioz Stars, 2026-09
    94/100 stars

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    Recombinant:

    Article Title: Differential regulation of proteoglycan 4 metabolism in cartilage by IL-1alpha, IGF-I, and TGF-beta1.
    Article Snippet: .. Recombinant human IL-1a, porcine TGF-b1, and recombinant human IGF-I were obtained from R&D Systems (Minneapolis, MN). ..

    Article Title: The in vitro response of human retinal endothelial cells to cytokines and other chemically active agents is altered by coculture with vitreous-derived hyalocytes.
    Article Snippet: Background: Ocular angiogenesis is regulated by polypeptides including cytokines, which are known to affect vascular endothelial cells.. We have reported that hyalocytes interact with vascular endothelial cells, and some cytokines affect these interactions.. Aims: To determine the effect of various chemically active agents on the viability of endothelial cells alone and cocultured with hyalocytes.

    Article Title: Regulation of IAPs gene family by interleukin-1 alpha and Epstein-Barr virus in nasopharyngeal carcinoma.
    Article Snippet: Background.. Inhibitors of apoptosis proteins (IAPs), which counteract apoptosis by potently inhibiting caspase activation, are promising targets of new anti-tumor therapy.. However, their roles in the pathogenesis of nasopharyngeal carcinoma (NPC), an Epstein-Barr virus (EBV)-associated carcinoma, are not fully understood.

    Article Title: Matrix metalloproteinase-3 secretion from human pancreatic periacinar myofibroblasts in response to inflammatory mediators.
    Article Snippet: Results: In human pancreatic myofibroblasts, MMP-3 secretion and mRNA expression were induced by interleukin (IL)-17, IL-1A, and tumor necrosis factor (TNF) Y>, respectively.. The effects of IL-17 were detected as similar in extent to those induced by IL-1A or TNF->.. Costimulation by IL-17 plus IL-1A and/or IL-17 plus TNF-> induced a synergistic increase in MMP-3 secretion, although the costimulatory effects of these combinations were not detected in tissue inhibitor of matrix metalloproteinaseY1 secretion.

    Article Title: Preconditioning with interleukin-1 alpha is required for the neuroprotective properties of mesenchymal stem cells after ischemic stroke in mice
    Article Snippet: .. MSCs were preconditioned with either recombinant human IL-1a (10 ng/ml, R&D Systems, UK) in serum free MesenPro RS medium or MesenPro alone for nonconditioned MSCs for 5min as previously described.6 The preconditioning medium was then removed and MSCs were washed twice with PBS before being dissociated with 0,5% trypsin-EDTA (Sigma-Aldrich, UK) and counted. ..

    Article Title: Influence of interleukin-1alpha on androgen receptor expression and cytokine secretion by cultured human dermal papilla cells.
    Article Snippet: Dermal papilla cells (DPC) control the growth character of the hair follicle through their elaboration of mitogenic factors and extracellular matrix components.. Further, the dermal papilla is a primary site of androgen action in the hair follicle.. Interleukin-1a (IL-1a) is prominent in skin wounding and inflammatory responses although regarded as a negative hair growth regulator.

    Article Title: Tocilizumab overcomes chemotherapy resistance in mesenchymal stem-like breast cancer by negating autocrine IL-1A induction of IL-6
    Article Snippet: All cell lines were cultured in DMEM (HyClone- Logan, Utah) supplemented with 10% fetal bovine serum (FBS), Antibiotic-Antimycotic (GenDepot- Katy, Texas), and L-glutamine (Corning- Manassas, Virginia) in a 5% CO 2 incubator at 37 °C. .. For the indicated in vitro conditions, the following tissue culture reagents were used at following concentrations unless noted differently: docetaxel (NovaPlus, 4 ng/ml from Ebewe Pharma, Austria), human IL-1A neutralizing antibody (R&D clone 4414, 1 μg/ml), mouse IgG 2A isotype control antibody (R&D clone 20102, 1 μg/ml), recombinant human IL-1A (R&D 200-LA, 2 ng/ml), DMSO (Sigma D2650), PD 0325901 (R&D 4192, 1 μM), human IL-6 neutralizing antibody (BD Biosciences clone MQ2-13A5, 0.1 μg/ml), rat IgG 1 isotype control antibody (BD Biosciences clone R3-34, 0.1 μg/ml), tocilizumab (Roche, 30 ng/ml from San Francisco, CA), and Kira6 (Cayman Chemical 19151 from Ann Arbor, MI). .. Anti-human antibodies used for ELISAs: IL-1A capture and detection (R&D clone 4414 and catalog# BAF200, respectively), IL-6 capture and detection (BD Biosciences clones MQ2-13A5 and MQ2-39C3, respectively), IL-8 capture and detection (BD Biosciences clones G265-5 and G265-8, respectively).

    Article Title: Critical role of IL‐1α in IL‐1β‐induced inflammatory responses: cooperation with NF‐κBp65 in transcriptional regulation
    Article Snippet: .. Recombinant human IL-1a (200-LA-002/CF) and IL-1b (201-LB) were purchased from R&D Systems (Minneapolis, MN, USA). .. Antibodies for p-TAK1(Thr184/187), TAK1(Ser412), p-IRAK4(Thr345/Ser346), p-IRAK1(Thr209), p-TAB2(Ser372), IL-1b, and IRAK4 were purchased from Cell Signaling Technology (90C7, 9339S, D6D7, T209, 8155, D3U3E, and 4363P; Danvers, MA, USA).

    In Vitro:

    Article Title: Tocilizumab overcomes chemotherapy resistance in mesenchymal stem-like breast cancer by negating autocrine IL-1A induction of IL-6
    Article Snippet: All cell lines were cultured in DMEM (HyClone- Logan, Utah) supplemented with 10% fetal bovine serum (FBS), Antibiotic-Antimycotic (GenDepot- Katy, Texas), and L-glutamine (Corning- Manassas, Virginia) in a 5% CO 2 incubator at 37 °C. .. For the indicated in vitro conditions, the following tissue culture reagents were used at following concentrations unless noted differently: docetaxel (NovaPlus, 4 ng/ml from Ebewe Pharma, Austria), human IL-1A neutralizing antibody (R&D clone 4414, 1 μg/ml), mouse IgG 2A isotype control antibody (R&D clone 20102, 1 μg/ml), recombinant human IL-1A (R&D 200-LA, 2 ng/ml), DMSO (Sigma D2650), PD 0325901 (R&D 4192, 1 μM), human IL-6 neutralizing antibody (BD Biosciences clone MQ2-13A5, 0.1 μg/ml), rat IgG 1 isotype control antibody (BD Biosciences clone R3-34, 0.1 μg/ml), tocilizumab (Roche, 30 ng/ml from San Francisco, CA), and Kira6 (Cayman Chemical 19151 from Ann Arbor, MI). .. Anti-human antibodies used for ELISAs: IL-1A capture and detection (R&D clone 4414 and catalog# BAF200, respectively), IL-6 capture and detection (BD Biosciences clones MQ2-13A5 and MQ2-39C3, respectively), IL-8 capture and detection (BD Biosciences clones G265-5 and G265-8, respectively).

    Control:

    Article Title: Tocilizumab overcomes chemotherapy resistance in mesenchymal stem-like breast cancer by negating autocrine IL-1A induction of IL-6
    Article Snippet: All cell lines were cultured in DMEM (HyClone- Logan, Utah) supplemented with 10% fetal bovine serum (FBS), Antibiotic-Antimycotic (GenDepot- Katy, Texas), and L-glutamine (Corning- Manassas, Virginia) in a 5% CO 2 incubator at 37 °C. .. For the indicated in vitro conditions, the following tissue culture reagents were used at following concentrations unless noted differently: docetaxel (NovaPlus, 4 ng/ml from Ebewe Pharma, Austria), human IL-1A neutralizing antibody (R&D clone 4414, 1 μg/ml), mouse IgG 2A isotype control antibody (R&D clone 20102, 1 μg/ml), recombinant human IL-1A (R&D 200-LA, 2 ng/ml), DMSO (Sigma D2650), PD 0325901 (R&D 4192, 1 μM), human IL-6 neutralizing antibody (BD Biosciences clone MQ2-13A5, 0.1 μg/ml), rat IgG 1 isotype control antibody (BD Biosciences clone R3-34, 0.1 μg/ml), tocilizumab (Roche, 30 ng/ml from San Francisco, CA), and Kira6 (Cayman Chemical 19151 from Ann Arbor, MI). .. Anti-human antibodies used for ELISAs: IL-1A capture and detection (R&D clone 4414 and catalog# BAF200, respectively), IL-6 capture and detection (BD Biosciences clones MQ2-13A5 and MQ2-39C3, respectively), IL-8 capture and detection (BD Biosciences clones G265-5 and G265-8, respectively).



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